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Methods Mol Biol 2010,Jan,01;576:99-133; (PMID: 19882260)
[...]) has become the most commonly used technique for identifying differentially expressed genes because of its many advantages over competing technologies such as DNA microarray, serial analysis of gene expression (SAGE), and subtractive hybridization. A large number of these publications have been in the field of [...]
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Hum Hered 2010,Jan,01;69(1):45-51; (PMID: 19797908)
DESPAIR (DESign for PAIRs) is a computer program useful for designing a two-stage linkage study using relative pairs for a dichotomous phenotype. It determines the optimal two-stage study design - i.e., for specified power and significance level, how many pairs of relatives should be studied, how many equally spaced markers should be used initially, and what criterion should be used to specify the markers around which further searching should be done at a second stage.[...]
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Accid Anal Prev 2010,Jan,01;42(1):1-9; (PMID: 19887138)
To investigate the relation between schools' resource levels (i.e., annual per student expenditures), school resource allocations, and physical assault (PA) against Minnesota's educators, a study was conducted from the two-phase Minnesota Educators' Study (MES) that incorporated school-level fiscal and demographic data from the Minnesota Department of Education (MDE). The MES examined a randomly selected cohort of employed, state-licensed kindergarten through grade 12 educators. From mailed questionnaires, response rates for both Phase I (comprehensive data collection on violent events) and Phase II (case-control) were 84%.[...]
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Methods Mol Biol 2010,Jan,01;576:351-62; (PMID: 19882271)
[...] has constantly evolved, with numerous technologies being available for RNA quantification, including differential display, serial analysis of gene expression (SAGE), quantitative real-time (qRT) polymerase chain reaction (PCR), and microarrays. Although every technique has its specific application, the high levels of accuracy, reproducibility, sensitivity, and specificity have established qRT-[...]
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Methods Mol Biol 2010,Jan,01;576:135-54; (PMID: 19882261)
[...] of the limited amount of messenger RNA (mRNA) isolated from the microdissected tissue in order to be able to proceed with comprehensive gene expression analyses via microarray or serial analysis of gene expression (SAGE) technology. Here we describe a manual microdissection strategy for the isolation of high-quality RNA. Furthermore, a strategy for combining linear [...]
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Vaccine 2009,Dec,09;27(52):7236-7; (PMID: 19450645)
[...]After a series of meetings and careful consideration of the evidence for HPV vaccine introduction, WHO's Strategic Advisory Group of Experts (SAGE) on vaccines and immunization recommended use of HPV vaccine in November 2008. WHO published a complete position paper in April 2009, which presents the WHO Position.[...]
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J Biol Chem 2009,Dec,04;284(49):33750-62; (PMID: 19815551)
Platelets are activated by adhesion to vascular collagen via the immunoglobulin receptor, glycoprotein VI (GPVI). This causes potent signaling toward activation of phospholipase Cgamma2, which bears similarity to the signaling pathway[...]
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Exp Eye Res 2009,Dec,01;89(6):967-71; (PMID: 19699197)
[...] worldwide. We studied the transcriptome of the diabetic retina using Series Analysis of Gene Expression (SAGE) technology and observed a 45.6% reduction in transcript levels of glutamine synthetase (GS[...]
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Clin Orthop Relat Res 2009,Dec,01;467(12):3051-62; (PMID: 19727989)
[...]. An accompanying biographical sketch of Marshall R. Urist, MD is available at DOI 10.1007/s11999-009-1067-4; a second Classic Article is available at DOI 10.1007/s11999-009-1069-2; and a third Classic Article is available at DOI 10.1007/s11999-009-1070-9. The Classic Article is copyright 1971 by Sage Publications Inc. Journals and is reprinted with permission from Urist MR, Strates BS. Bone morphogenetic protein. J Dent Res. 1971;50:1392-1406.
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Radiother Oncol 2009,Dec,01;93(3):474-8; (PMID: 19758720)
BACKGROUND AND PURPOSE: Accurate conformal radiotherapy treatment requires manual delineation of target volumes and organs at risk (OAR) that is both time-consuming and subject to large inter-user variability. One solution is atlas-based automatic segmentation (ABAS) where a priori information is used to delineate various organs of interest.[...]
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