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J Cell Physiol 2010,Oct,01;225(1):92-105; (PMID: 20648627)
[...]Accordingly, DAB2 was identified as a plakoglobin-binding partner with the interaction mediated by the phosphotyrosine binding domain of DAB2 and the Asn-Pro-Asp-Tyr (NPDY) motif of plakoglobin. Molecular analysis and transcriptome profiling also revealed that DAB2 was involved in the regulation of insulin-like growth factor 2-mediated [...]
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Toxicology 2010,Sep,10;275(1-3):72-8; (PMID: 20561557)
[...], which can be detected using the phosphotyrosine-specific antibody PY-20 or 4G10, is significantly increased after treatment with TCDD. In addition, pretreatment of the cells with genistein, a protein tyrosine kinase inhibitor, abolished the [...]
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Anal Biochem 2010,Sep,01;404(1):64-74; (PMID: 20447373) [FULL TEXT AVAILABLE]
[...]CBDP, adding a mass of 170amu to Tyr411 to yield the o-cresyl phosphotyrosine derivative. Human butyrylcholinesterase made a covalent bond with CBDP on Ser198 to yield five adducts with added masses of 80, 108, 156, 170, and 186amu.[...]
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Eur J Cell Biol 2010,Sep,01;89(9):674-80; (PMID: 20537759)
[...]-based matrix display invadopodia which are formed by a thick protruding base rich in F-actin, phospho-paxillin, phospho-cortactin and phosphotyrosine signal, from which numerous thin filaments protrude into the matrix. The protruding filaments are composed of an F-actin core and are free of phospho-paxillin and phospho-cortactin but capped by [...]
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J Immunol 2010,Sep,01;185(5):2819-27; (PMID: 20668219)
[...], a residue previously implicated in Fyn recruitment, yet SAP's interactions with PKC-theta occurred independent of phosphotyrosine binding and Fyn. Overexpression of SAP in T cells increased and sustained PKC-theta recruitment to the immune synapse and elevated [...]
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Invest Ophthalmol Vis Sci 2010,Sep,01;51(9):4722-31; (PMID: 20357199)
[...]Transient or sustained activation of TrkB receptors in vivo was quantified by Western blot analysis retinal samples for TrkB-phosphotyrosine. Time-dependent changes to the neuronal retinal layers were quantified longitudinally by Fourier domain-optical coherence tomography.[...]
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J Am Chem Soc 2010,Aug,18;132(32):11058-70; (PMID: 20698672)
[...]molecular dynamics (MD) simulations were used to probe the structure and dynamics of complexes of three phosphotyrosine-derived peptides with the Src SH2 domain in an effort to uncover a structural explanation for enthalpy-entropy compensation observed in the binding thermodynamics.[...]
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Neurosci Lett 2010,Aug,09;480(1):35-9; (PMID: 20570712) [FULL TEXT AVAILABLE]
[...] phosphorylated procaspase-9 and Apaf-1 were determined by immunoblotting using specific anti-procaspase-9, anti-Apaf-1 and anti-phosphotyrosine antibodies. ATP levels (mumoles/g brain) were 4.3+/-0.2 in the Nx and 1.4+/-0.3 in the Hx and 1.7+/-0.3 in Hx+nNOS I group (p<0.05 vs. Nx) groups.[...]
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J Proteome Res 2010,Aug,06;9(8):4113-22; (PMID: 20568816)
[...]Here, we use peptide pull-down assays and quantitative mass spectrometry to identify interaction partners of site-specifically phosphorylated ADAP sequences. Phosphotyrosine peptide motifs covering Y595, Y625, and Y771 and the corresponding nonphosphorylated sequences were covalently coupled to [...]
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Oncogene 2010,Aug,05;29(31):4449-59; (PMID: 20498643)
[...]) cell lines, supporting a role for VEGF in HNSCC tumorigenesis. Using a phosphotyrosine proteomics approach, we screened the HNSCC cell line, squamous cell carcinoma-9 for effectors of VEGFR2 signaling. A cluster of proteins involved in [...]
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